A stable isotope approach to accurately determine iron and zinc bioaccessibility in cereals and legumes based on a modified INFOGEST static in vitro digestion method

The establishment of the INFOGEST in vitro static digestion method, a standardized international consensus, was an important milestone field food digestion. We evaluated contribution iron and zinc reagents used method relation to sample potential interference reagent-derived with bioaccessibility measurements. In most cases, contributed more than 50% total or digesta containing selected cereals legumes. Moreover, chemical behaviour matrix dependent such that application blanket blank correction not appropriate. therefore propose improved approach involving isotopic labelling reagent order discriminate between sample-derived each matrix. This stable isotope could improve accuracy reliability studies.